Anti-Zyxin Monoclonal Antibody

Zyxin antibody

Boster Bio Anti-Zyxin Monoclonal Antibody catalog # M02365. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: M02365
Size: 100 μl
Reactive Species: Human, Mouse
Host: Rabbit
Application: Flow Cytometry, IP, IF, IHC, ICC, WB

Product Name

Anti-Zyxin Monoclonal Antibody

View all Zyxin Antibodies

SKU/Catalog Number

M02365

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Zyxin Monoclonal Antibody catalog # M02365. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Zyxin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02365)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AFOG-26

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Zyxin.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M02365 is reactive to ZYX in Human, Mouse

Applications

M02365 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

55 kDa

Calculated molecular weight

61.277kDa

Background of Zyxin

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:500

Validation Images & Assay Conditions

Gene/Protein Information For ZYX (Source: Uniprot.org, NCBI)

Gene Name

ZYX

Full Name

Zyxin

Weight

61.277kDa

Superfamily

zyxin/ajuba family

Alternative Names

CTCL tumor antigen se14-3; CTCL-associated antigen se14-3; cutaneous T-cell lymphoma associated antigen se14-3; Cutaneous T-cell lymphoma-associated antigen se14-3; KIAA1125; MGC31836; predicted protein of HQ2893; PRKCBP1; PRO2893; protein kinase C-binding protein 1; Rack7; RACK7protein kinase C binding protein 1; zinc finger MYND domain containing protein 8; Zinc finger MYND domain-containing protein 8; zinc finger, MYND-type containing 8; Zyx; Zyxin 2; Zyxin ZYX ESP-2, HED-2 zyxin zyxin|zyxin-2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ZYX, check out the ZYX Infographic

ZYX infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ZYX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-Zyxin Monoclonal Antibody

Question

Do you have a BSA free version of anti-Zyxin Monoclonal antibody M02365 available?

Verified Customer

Verified customer

Asked: 2020-04-08

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Zyxin Monoclonal antibody M02365 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-04-08

Question

Will M02365 anti-Zyxin Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-03-23

Answer

It shows on the product datasheet, M02365 anti-Zyxin Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-03-23

Question

What sample was tested in WB and IHC applications with M02365?

Verified customer

Asked: 2020-01-09

Answer

Western blot analysis of Zyxin expression in MCF7 cell lysate.

Boster Scientific Support

Answered: 2020-01-09

Question

Would anti-Zyxin Monoclonal antibody M02365 work on horse IP with colon carcinoma?

Verified Customer

Verified customer

Asked: 2019-09-23

Answer

Our lab technicians have not tested anti-Zyxin Monoclonal antibody M02365 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Zyxin Monoclonal antibody M02365 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse colon carcinoma in IP, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-09-23

Question

Does anti-Zyxin Monoclonal antibody M02365 work for IP with colon carcinoma?

Verified Customer

Verified customer

Asked: 2018-12-05

Answer

According to the expression profile of colon carcinoma, ZYX is highly expressed in colon carcinoma. So, it is likely that anti-Zyxin Monoclonal antibody M02365 will work for IP with colon carcinoma.

Boster Scientific Support

Answered: 2018-12-05

Question

We are currently using anti-Zyxin Monoclonal antibody M02365 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on canine tissues as well?

M. Wu

Verified customer

Asked: 2016-02-01

Answer

The anti-Zyxin Monoclonal antibody (M02365) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-02-01

Order DetailsPrice
M02365

100μL

$370
M02365-30ul

30μL sample

$99
M02365-carrier-free

Carrier Free

$370

More conjugates/formats

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M02365
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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